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To reduce irritation and lumps, it helps to rotate injection sites (dont inject the same spot repeatedly), inject into healthy skin (avoid bruised, scarred, inflamed, or irritated areas), and keep your routine consistent (many protocols are taken in the morning)

A few small human trials have been conducted, primarily for inflammatory bowel disease

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doi: 10.3390/pharmaceutics17111484, 145

The Mayo Clinic says that although side effects from taking folic acid are rare, they are possible